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Record W1489089330

Threshold for Positional Weight Matrix.

2008· article· en· W1489089330 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

fundA Canadian funder is recorded on the work.
venuePublished in a venue whose home country is Canada.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueNPARC · 2008
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicFractal and DNA sequence analysis
Canadian institutionsnot available
FundersNational Research Council Canada
KeywordsComputer scienceMatrix (chemical analysis)Materials scienceComposite material
DOInot available

Abstract

fetched live from OpenAlex

Abstract—In biological sequence research, the positional weight matrix (PWM) is often used to search for putative transcription factor binding sites. A set of experimentally verified oligonucleotides known to be functional motifs are collected and aligned. The frequency of each nucleotide A, C, G, or T at each column of the alignment is calculated in the matrix. Once a PWM is constructed, it can be used to search from a nucleotide sequence for subsequences that can possibly perform the same function. The match between a subsequence and a PWM is usually described by a score function, which measures the closeness of the subsequence to the PWM as compared with the given background. Nevertheless, the score function is usually motif-length-dependent and thus there is no universally applicable threshold. In this paper, we propose an alternative scoring index (G) varying from zero, where the subsequence is not much different from the background, to one, where the subsequence fits best to the PWM. We also propose a measure evaluating the statistical expectation at each G index. We investigated the PWMs from the TRANSFAC and found that the statistical expectation is significantly (p<0.0001) correlated with both the length of the PWMs and the threshold G value. We applied this method to two PWMs (GCN4_C and ROX1_Q6) of yeast transcription factor binding sites and two PWMs (HIC1-02, HIC1_03) of the human tumor suppressor (HIC-1) binding sites from the TRANSFAC database. Finally, our method compares favorably with the broadly used Match method. The results indicate that our method is more flexible and can provide better confidence.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.143
Threshold uncertainty score0.221

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.252
Teacher spread0.240 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it