<i>Agrobacterium</i>-mediated Transformation of Cotton Shoot Apex with <i>SNC1</i> Gene and Resistance to Cotton Fusarium Wilt in T<sub>1</sub> Generation
Why this work is in the frame
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Bibliographic record
Abstract
Using shoot apexes from the upland cotton varieties, named Zhong 35 and Junmian No.1 as explant, the SNC1 , suppressor of npr1-1constitutive cloned from Arabidopsis thaliana , was introduced into cotton genome by Agrobacterium -mediated transformation with the optimized transformation conditions including culture stage of the explant, infection time and co-culture time. The results showed that the transformation procedure for high transformation rate was as follows, in vitro cuture the explants one day, infecting 20 min and co-culturing two days. PCR and RT-PCR identification of T 0 and T 1 regeneration plants revealed that the SNC1 gene was successfully integrated into cotton genome and expressed in the transgenic plant. Furthermore, comparing with non-transgenic plants, the T 1 generation transgenic plants acquired the significant resistance to the inoculation of with the Fusarium oxysporum f.sp. Vasinfectum by the validation of the root-dipping method, which suggested that the SNC1 should facilitate the resistant to cotton Fusarium wilt.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it