Lysozyme transgenic Atlantic salmon (Salmo salar L.)
Bibliographic record
Abstract
Disease outbreaks are a major constraint to salmon aquaculture worldwide. Therefore, there is considerable interest in finding means to alleviate this problem by enhancing their innate immune system. Because lysozyme is an important component of this system, we generated a line of transgenic Atlantic salmon using a gene construct consisting of a rainbow trout lysozyme gene under the control of the ocean pout antifreeze protein gene promoter op5a (opAFP-rtLys). The results show that the transgene exhibited Mendelian inheritance at both the F1 and the F2 generations and demonstrated that it had integrated into a single chromosome in the genetic founder. Sequence analysis of F2 generation salmon revealed the presence of a complete copy of an intact lysozyme transgene integrant that was identical in sequence to that of the construct. The computationally translated peptide sequence of the lysozyme coding region differed by four amino acids from those present in GenBank (accession nos CAA42084; AAG34564). Reverse transcriptase-polymerase chain reaction analysis demonstrated the expression of the transgene in most body tissues. However, Northern analysis revealed that visible levels of mRNA were only evident in the spleen, skin and kidney. The lysozyme lytic activity of kidney tissue extracts from F2 transgenic salmon was 40% greater than it was in non-transgenic siblings.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".