<i>PolA2</i>is required for embryo development in<i>Arabidopsis</i>This paper is one of a selection of papers published in a Special Issue from the National Research Council of Canada – Plant Biotechnology Institute.
Bibliographic record
Abstract
DNA replication machinery is highly conserved in eukaryotes. DNA polymerase is essential for the synthesis of new DNA strands and for DNA repair. Despite the significant progress in the understanding of these processes in yeast and animal model systems, there is only scant information available for their counterparts in plants. Among different multisubunit-containing DNA polymerases, DNA polymerase α (POLA complex) is composed of four subunits. In this study, we report on the characterization of PolA2, which encodes the putative B subunit of DNA polymerase α in Arabidopsis thaliana (L.) Heynh. PolA2 is a single copy gene in Arabidopsis and shows highly conserved regions with putative homologs in other plant species. Insertional inactivation of PolA2 in Arabidopsis leads to embryo lethality, with developmental arrest at or before the four-cell stage during embryogenesis. The apical cell lineage is strongly affected in the mutant embryos and the endosperm initial cell fails to divide. PolA2 is expressed broadly in the early phases of embryo development during the period of active cell divisions, while during the later stages of development expression is reduced and more localized. Ectopic overexpression of PolA2 produced dominant negative phenotypes with gametic and embryo lethality suggesting that coordinated and parallel expression with other subunits is critical for its proper function in DNA replication and plant development.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".