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Record W1502135961 · doi:10.1002/047001153x.g404302

Phylogenetic analysis of <scp>BLAST</scp> results

2005· other· en· W1502135961 on OpenAlexaff
Fiona S. L. Brinkman

Bibliographic record

VenueEncyclopedia of Genetics, Genomics, Proteomics and Bioinformatics · 2005
Typeother
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Phylogenetic Studies
Canadian institutionsSimon Fraser University
Fundersnot available
KeywordsPhylogenetic treeIdentification (biology)Perspective (graphical)Sequence (biology)Variety (cybernetics)Computer scienceSequence analysisPhylogeneticsComputational biologyBiologyArtificial intelligenceGeneGenetics

Abstract

fetched live from OpenAlex

Abstract BLAST (Basic Local Alignment Search Theorem) is one of the most elegant, and widely used bioinformatics analysis developed to date. Like many bioinformatic analyses, BLAST uses evolutionary theory as the basis for its assumptions. Therefore, an understanding of evolutionary theory is critical to the appropriate interpretation, and further analysis, of BLAST results. Viewing essentially one‐dimensional BLAST analysis from the perspective of a two‐dimensional phylogenetic analysis has a number of benefits including more accurate identification of the true “top hit”, delineation of gene families, identification of true homologs, and improved functional assignment of orthologs and paralogs. Performing such phylogenetic analyses has become easier, now that semiautomated methods have been developed that permit rough phylogenetic overviews of the data. However, it should be emphasized that phylogenetic analysis must often be customized for a given experiment or research question. Such issues are relevant not only to the further analysis of a BLAST output, but also to similar analyses of outputs from other widely used algorithms for rapid database search. Critical analysis of results is becoming increasingly important as sequence databases increase in both size and complexity – and as we begin to understand that even these large sequence databases are only scratching the surface of the true complexity and variety of sequences that exist in nature.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.007
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.021
Threshold uncertainty score0.069

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0020.007
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0060.008
Science and technology studies0.0010.000
Scholarly communication0.0020.001
Open science0.0010.001
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0210.010

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.214
Teacher spread0.208 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2005
Admission routes1
Has abstractyes

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