Isolation and Expression Analysis of a β-Ketoacyl-Acyl Carrier Protein Synthase â… gene from <i>Arachis hypogaea</i> L.
Bibliographic record
Abstract
β-ketoacyl-acyl carrier protein synthase (KAS) plays a pivotal role in de novo fatty acid biosynthesis, joining short carbon units to construct fatty acyl chains by a three-step Claisen condensation reaction in plants and bacteria. A putative KASâ… cDNAs was isolated from peanut by searching a peanut seedling full-length cDNA library. The AhKASâ… contains four strictly conserved residues Cys221–His361–Lys392–His397 in the active site, which is an important characteristic of KAS Is in plants and bacteria. The AhKAS â… gene containing 1 912 bp cDNA sequence with a 1 413 bp ORF, encodes 470 amino acids. The predicted amino acid sequences translated from the AhKASâ… gene shared 90.2% sequence identity to the corresponding protein in Glycine max . The cDNA was cloned into plasmid pET-28a and expressed in Escherichia coli BL21. Quantitative real-time PCR analysis suggested AhKAS â… was expressed with higher levels in leaf and seed than those in other tissues. In addition, AhKAS â… RNA was found in high abundance at 45 and 65 DAP (days after pegging) during seed development. This work may serve as a foundation for further studies on the mechanisms regulating the expression of KAS â… gene and provide candidate genes for modifying oil quality via transgenic plants.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".