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Record W1509977047 · doi:10.1002/0471683779.ch2

Metal‐Labeled DNA on Surfaces

2004· other· en· W1509977047 on OpenAlexaff
Heinz‐Bernhard Kraatz, Yi‐Tao Long, Todd C. Sutherland

Bibliographic record

Venuenot available
Typeother
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicAdvanced biosensing and bioanalysis techniques
Canadian institutionsUniversity of Saskatchewan
Fundersnot available
KeywordsDNAMetalChemistryComputational biologyMaterials scienceBiologyBiochemistryMetallurgy

Abstract

fetched live from OpenAlex

The self-assembly properties of DNA have been exploited to generate 2D and 3D structures on surfaces and in solution. DNA now can be modified by metal centers at individual bases or at either termini. The ability to label DNA sites specifically with metal centers allows us to influence the electronic properties of the assembly. Potential applications of these modified DNA constructs in the design of nanoelectronic or bioelectronic circuitry may be within reach. Especially, the most recent developments in the area of direct basepair metallation, such as Cu-DNA and M-DNA, allow superb control over the electronics of the DNA construct and reduce the synthetic efforts. As shown in this review, a large driving force for research in the area of metal-DNA conjugates stems from sensor applications, for example in the detection of genetic defects, genomic fingerprinting for identification purposes, or applications in personalized medicine. Electrochemical detection methods offer a superior sensitivity to currently available optical gene chip technology. Gene chip technology has to rely on attaching a fluorescence tag to one of the DNA strands after PCR amplification. Recent advances in the area of electrochemical DNA detection described in this review should allow the development of biosensors that do not require labeling of the target DNA. One of the holy grails of E-biosensors is the detection of single nucleotide mismatches time- and cost-effectively without the need for a lengthy PCR amplification, or in heterozygote mixtures or under non-ideal hybridization conditions. These goals are yet unrealized. Especially difficult will be the detection of DNA under “real-to-life” conditions, such as high salt concentrations or in the presence of large quantities of impurities. However, the possibility of single molecule detection using ultra-fast electrochemical techniques on ultramicroelectrodes may offer solutions to this problem.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.007
Threshold uncertainty score0.023

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.001
Open science0.0010.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0070.004

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.264
Teacher spread0.256 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2004
Admission routes1
Has abstractyes

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