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Record W1513044259 · doi:10.1007/978-1-60761-292-6_5

Isolation and Culture of Primary Human CNS Neural Cells

2009· book-chapter· en· W1513044259 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueSpringer protocols handbooks/Springer protocols · 2009
Typebook-chapter
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRNA Research and Splicing
Canadian institutionsUniversity of CalgaryMontreal Neurological Institute and Hospital
FundersCanadian Institutes of Health Research
KeywordsNeurosphereHippocampal formationIsolation (microbiology)Primary cultureBiologyCell cultureNeuroscienceCell biologyIn vitroBioinformaticsAdult stem cellGenetics

Abstract

fetched live from OpenAlex

We present our current methods for isolating and culturing cells from the adult and fetal human CNS. The cell isolation procedures used are also well suited to obtain samples for immediate RNA- and protein-based analyses, particularly as techniques to minimize the amount of material needed become increasingly available. Further tissue culture techniques commonly used for rodent material can be adapted to human adult and fetal tissues such as the hippocampal slice culture and the neurosphere assay (Dorr et al. J Neuroimmunol 167:204–209, 2005; Chojnacki et al. Ann Neurol 64:127–142, 2008).

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Protocol · Consensus signal: Protocol
Teacher disagreement score0.444
Threshold uncertainty score0.999

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.029
GPT teacher head0.305
Teacher spread0.276 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it