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Record W151453900 · doi:10.1023/a:1005559317975

Sendai Virus Binds to a Dispersed Population of NBD-GD1a

2000· article· en· W151453900 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueBioscience Reports · 2000
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicLipid Membrane Structure and Behavior
Canadian institutionsWestern University
Fundersnot available
KeywordsSendai virusFluorescence correlation spectroscopyPopulationBiophysicsVirusMembraneGangliosidePhotobleachingViral envelopeConfocal microscopyConfocalBiologyChemistryCell membraneCell biologyBiochemistryVirologyFluorescencePhysics

Abstract

fetched live from OpenAlex

Receptor aggregation is believed to be an important step in the attachment of membrane enveloped virus' to target cell membranes. A likely receptor for Sendai virus is the ganglioside GD1a. In this work we have studied the membrane diffusion of the fluorescent ganglioside NBD-GD1a on the surface of CV-1 cells with standard photobleaching techniques. Using confocal laser scanning microscopy (CLSM) and Image Correlation Spectroscopy (ICS) NBD-GD1a is shown to exist in at least two populations: dispersed and aggregated. By quantifying the distribution of NBD-GD1a pre- and post-incubation with Sendai virus it is shown that the virus induces a dose-dependent clustering of NBD-GD1a. Image cross-correlation spectroscopy (ICCS) is used to further quantitatively characterize this clustering by demonstrating that it occurs due to binding of virus to the dispersed population of NBD-GD1a.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.106
Threshold uncertainty score0.320

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.258
Teacher spread0.251 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it