Soybean peroxidase trapped in product precipitate during phenol polymerization retains activity and may be recycled
Why this work is in the frame
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Bibliographic record
Abstract
Abstract BACKGROUND Studies with peroxidase (EC 1.11.1.7) have demonstrated that phenolic precipitate adsorbs free enzyme, inactivating it. The end‐product inactivation model has been proposed to explain the effect. Additives, such as polyethylene glycol and Triton X‐100, were reported to effectively extend enzyme lifetime by preventing the adsorption . RESULTS It was found that soybean peroxidase ( SBP , EC 1.11.1.7) trapped in precipitate during phenol polymerization retains activity. Contrary to the end‐product inactivation model, recycling precipitate effectively utilized the active SBP . The minimum SBP concentration required for the subsequent batch reaction removal of 1 mmol L ‐1 phenol from aqueous solution was reduced from 1.2 to 0.5 U mL ‐1 . SBP adsorption on the precipitate was proven to be reversible by the addition of Triton X‐100. Thus, a new explanation of SBP fate during the reaction is suggested: SBP is immobilized in situ in an active form with reduction of specific activity rather than inactivation. The adsorption is characterized by a Langmuir isotherm . CONCLUSIONS The phenolic precipitate immobilizes SBP in an active form, consistent with the Langmuir isotherm model. Recycling the precipitate improves the enzyme economy in phenol removal. © 2013 Society of Chemical Industry
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it