MétaCan
Menu
← Back to cohort
Record W1535796923 · doi:10.18174/199007

Development and application of in vitro and in vivo reporter gene assays for the assessment of (xeno-)estrogenic compounds in the aquatic environment

2001· dissertation· en· W1535796923 on OpenAlexfundno aff
Juliette Legler

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldEnvironmental Science
TopicEffects and risks of endocrine disrupting chemicals
Canadian institutionsnot available
FundersMichigan State UniversityStichting voor de Technische WetenschappenRijksinstituut voor Volksgezondheid en MilieuNatural Sciences and Engineering Research Council of CanadaCollege of Engineering, Michigan State UniversityUniversiteit van Amsterdam
KeywordsBioassayReporter geneLuciferaseIn vivoIn vitroIn vitro toxicologyEstrogen receptorXenobioticBiologyEstrogenEndocrine systemOryziasChemistryPotencyEC50TransfectionPharmacologyGene expressionGeneBiochemistryHormoneEndocrinologyBiotechnologyGeneticsCancer

Abstract

fetched live from OpenAlex

Chapter 2Development of a stably transfected estrogen receptor-mediated luciferase reporter gene assay in the human T47D breast cancer cell line 17 Chapter 3Detection of estrogenic activity in sediment-associated compounds using in vitro reporter gene assays 39 Chapter 4Measurement of estrogenic activity in human urine and fish bile using the in vitro ER-CALUX reporter gene assay 57 Chapter 5A novel in vivo bioassay for (xeno-)estrogens using transgenic zebrafish 71 Chapter 6Value of in vivo transgenic zebrafish and in vitro reporter gene assays for determination of estrogenic activity in environmental matrices 87 Chapter 7General Discussion (summary and concluding remarks)

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.005
Threshold uncertainty score0.017

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0010.000
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0050.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.322
Teacher spread0.312 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations10
Published2001
Admission routes1
Has abstractyes

Explore more

Same topicEffects and risks of endocrine disrupting chemicals→French-language works237,207→