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Record W1544911917 · doi:10.7202/008907ar

New insights in to ancient resistance: the molecular side of cell wall appositions

2004· article· en· W1544911917 on OpenAlexaffvenue
David L. Greenshields, Guosheng Liu, Gopalan Selvaraj, Yangdou Wei

Bibliographic record

VenuePhytoprotection · 2004
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicPlant-Microbe Interactions and Immunity
Canadian institutionsPlant Biotechnology InstituteUniversity of Saskatchewan
Fundersnot available
KeywordsBiologyPowdery mildewEpidermis (zoology)cDNA libraryBlumeria graminisCell biologyGeneCell wallPlant disease resistanceComplementary DNABotanyGeneticsAnatomy

Abstract

fetched live from OpenAlex

The epidermis lies at the interface between a plant and its environment. As such, the epidermis is crucial for protecting the plant against environmental insults. We focus primarily on cell wall reinforcement-mediated penetration resistance (papilla-resistance) against fungal pathogen attack. The epidermal cell layer of cereal leaves is the only tissue interacting with the powdery mildew fungus, Blumeria graminis , and papilla formation at sites of fungal penetration attempts provides a basal resistance, hampering fungal invasion irrespective of host specific compatibility or incompatibility. To elucidate the genetic scaffolding of penetration resistance mechanisms, we constructed a cDNA library from wheat leaf epidermis at 24-48 h post inoculation with B. graminis f. sp. tritici . We have sequenced 3,000 expressed sequence tags (ESTs) from this cDNA library. EST analysis revealed a large proportion of genes involved in plant defense/stress responses (1/3) and a low frequency of “house-keeping” genes. Enrichment of defense genes from this EST collection has allowed us to identify several defense and signaling pathways that have been hitherto poorly characterized, including cell wall biosynthesis, vesicle trafficking, redox regulation and metal homeostasis. Our results suggest that a global analysis of transcripts from this epidermis-specific cDNA library makes it feasible to define a full set of genes involved in early plant resistance associated with cell wall modifications.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.065
Threshold uncertainty score0.572

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.206
Teacher spread0.195 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations10
Published2004
Admission routes2
Has abstractyes

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