Abstract 17087: A Unique Calcium Channel Blocker Attenuates Myocardial Fibrosis and Prevents Human Cardiac Myofibroblast Activation Independent of Hemodynamics
Bibliographic record
Abstract
Introduction: Myocardial fibrosis is mediated by myofibroblasts that alter extracellular matrix (ECM). Tetrandrine (TTD) is a calcium channel blocker (CCB) with documented anti-fibrotic actions believed to be secondary to its effects on lowering blood pressure. In this study, for the first time, we identified that TTD can directly prevent in vitro human cardiac fibroblast activation and limit in vivo myocardial fibrosis independent of hemodynamic load. Methods and Results: IN VITRO: Cardiac fibroblasts from human atrial biopsies (N=10) were seeded in 3D collagen matrices. Cell-collagen constructs were exposed to TGF-β1 (10 ng/mL), with or without TTD (5μM) for 48 hours. Collagen gel contraction, myofibroblast activation (α-SMA expression), expression of pro-fibrotic mRNAs and rate of collagen protein synthesis was compared. TTD decreased collagen gel contraction (79.7±1.3 vs 60.1±8.9%, P< 0.01). Cell viability was similar between groups (annexin positive cells: 1.7% vs 1.4%). TTD decreased α-SMA expressio...
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".