Slowed muscle oxygen uptake kinetics with raised metabolism are not dependent on blood flow or recruitment dynamics
Bibliographic record
Abstract
Key points A slow adjustment of skeletal muscle oxygen uptake ( ) to produce energy during exercise predisposes to early fatigue. In human studies, kinetics are slow when exercise is initiated from an elevated baseline; this is proposed to reflect slow blood flow regulation and/or recruitment of muscle fibres containing few mitochondria. To investigate this, we measured kinetics in canine muscle, with experimental control over muscle activation and blood flow. We found that kinetics remained slow when contractions were initiated from an elevated baseline despite experimentally increased blood flow and uniform fibre activation. These data challenge our current understanding of the control of muscle and demand consideration of new alternative mediators for control. Abstract Oxygen uptake kinetics (τ ) are slowed when exercise is initiated from a raised metabolic rate. Whether this reflects the recruitment of muscle fibres differing in oxidative capacity, or slowed blood flow ( ) kinetics is unclear. This study determined τ in canine muscle in situ , with experimental control over muscle activation and during contractions initiated from rest and a raised metabolic rate. The gastrocnemius complex of nine anaesthetised, ventilated dogs was isolated and attached to a force transducer. Isometric tetanic contractions (50 Hz; 200 ms duration) via supramaximal sciatic nerve stimulation were used to manipulate metabolic rate: 3 min stimulation at 0.33 Hz (S1), followed by 3 min at 0.67 Hz (S2). Circulation was initially intact (SPON), and subsequently isolated for pump‐perfusion (PUMP) above the greatest value in SPON. Muscle was determined contraction‐by‐contraction using an ultrasonic flowmeter and venous oximeter, and normalised to tension‐time integral (TTI). τ /TTI and τ were less in S1 SPON (mean ± s.d.: 13 ± 3 s and 12 ± 4 s, respectively) than in S2 SPON (29 ± 19 s and 31 ± 13 s, respectively; P < 0.05). τ /TTI was unchanged by pump‐perfusion (S1 PUMP , 12 ± 4 s; S2 PUMP , 24 ± 6 s; P < 0.001) despite increased O 2 delivery; at S2 onset, venous O 2 saturation was 21 ± 4% and 65 ± 5% in SPON and PUMP, respectively. kinetics remained slowed when contractions were initiated from a raised metabolic rate despite uniform muscle stimulation and increased O 2 delivery. The intracellular mechanism may relate to a falling energy state, approaching saturating ADP concentration, and/or slowed mitochondrial activation; but further study is required. These data add to the evidence that muscle control is more complex than previously suggested.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".