Role of adaptive immune cells in the anti‐colitic effect of helminth antigen‐pulsed dendritic cells (650.17)
Bibliographic record
Abstract
Role of adaptive immune cells in the anti‐colitic effect of helminth antigen‐pulsed dendritic cells Chelsea E. Matisz 1 , Arthur Wang 1 , Keith A. Sharkey 1 , Derek M. McKay 1 . Department of Physiology & Pharmacology, University of Calgary, AB, Canada. Helminth parasites have evolved as potent modulators of the host immune system. Epidemiological, experimental, and clinical trials report that infection with helminths can attenuate concomitant inflammatory disease. The role of dendritic cells (DC) in these protective immune responses has only recently been explored. The adoptive transfer of in vitro derived dendritic cells, when pulsed with antigens from the helminth Hymenolepis diminuta (HD), protect recipient mice against dinitrobenzene sulfonic acid (DNBS)‐induced colitis. The transfer of HD‐pulsed DCs (HD‐DC) is associated with reduced disease activity scores, colonic myeloperoxidase (MPO) activity, improved histology damage scores, and increased IL‐4 and IL‐10 in stimulated splenocytes compared to DNBS only controls. As these cytokines are indicative of Th2 responses, we assessed the role of adaptive immunity in the protective effect of HD‐DCs. DCs were generated in vitro , treated with HD antigen for 24 hours, and transferred (10 6 , ip) to wild type and RAG deficient mice, which lack T and B cells. DNBS colitis was induced 48 hours after cell transfer, and necropsies performed 3 days later. Significantly less DNBS‐induced disease was observed in WT animals treated with HD‐DCs, compared to RAG deficient mice treated with HD‐DCs (n=6‐12, 2 experiments; F( 2,39 )=8.45, p=0.001). HD‐DCs significantly suppressed colonic MPO in both WT and RAG deficient mice. These results suggest that while T and/or B cells are essential to the anti‐colitic clinical response driven by HD‐DC, they are not required for reduced granulocyte infiltration. Overall these experiments highlight the therapeutic value of helminth antigens, and their potential to program DCs to attenuate inflammatory disease.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".