DECIPHERING THE ROLE OF A MICROTUBULE SEVERING PROTEIN AND A PROTEIN KINASE IN CELL CYCLE AND CILIOGENESIS IN CHLAMYDOMONAS REINHARDTII
Bibliographic record
Abstract
Many types of eukaryotic cells are ciliated – they project a microtubule-based structure extending from the cell surface called a cilium, which plays roles in cellular motility and sensing the environment. Cilia are anchored at the cell surface by microtubule-based structures called basal bodies or centrioles. In addition to nucleating cilia, centrioles act as spindle organizing centres during cell division. We are beginning to understand that several proteins are involved in simultaneously regulating cilia and centrioles with cell division. My research focused on the function of two proteins in the ciliated alga, Chlamydomonas. The first protein, katanin, severs microtubules and we predicted that it is required during the disassembly of cilia prior to mitosis. I used a genetic approach to repress expression of the gene encoding katanin in Chlamydomonas , and thereby demonstrated that katanin in fact severs microtubules at a distinct location between basal bodies and the cilium. The other gene that I studied encodes a cyclic GMP-dependent kinase type 2 (PKG2). Mutant cells deficient in PKG2 had either no cilia, or cilia of unequal length. I discovered that PKG2 is expressed in the cytoplasm as well as the flagella and may interact with other proteins to regulate ciliary length and structure. Taken together, this research identified novel mechanisms that help explain the coordinated regulation of cilia during the cell cycle, and a novel gene with roles in ciliary assembly was also identified.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".