DETECTION OF AROMATASE IN HUMAN ENDOMETRIAL TISSUE CULTURED IN THREE-DIMENSIONAL FIBRIN MATRIX IN VITRO
Bibliographic record
Abstract
Background: Endometriosis is the presence of endometrial glandular and stromal cells outside of the uterine cavity. Our previous study showed that in vitro culture of human endometrial tissue in a three-dimensional (3D) fibrin matrix could mimic the early stages of endometriosis with invasion, gland and stroma formation and sprouting of new vessels. Objective: The objective of the present study was to evaluate the expression of aromatase in in vitro cultured endometrial fragments. Materials and Methods: Endometrial samples obtained from the fundus of the uterine cavity of ten normal ovulating women on cycle days19-24 that referred to Toronto Center for Advanced Reproductive Technology (T.C.A.R.T) for infertility treatment and cultured in vitro by a three-dimensional fibrin matrix culture system. Cultures were performed in 24-well culture plates. Results: After 4 weeks, the tissues were fixed and blocked for immunostaining. They were stained by anti-aromatase antibodies. Although a fine anti-glycodelin positive glandular epithelium and anti-cyclooxygenase-2 (COX-2) positive new vessels were detected and reported in our previous study, we were unable to detect any aromatase activity in the cultured fragments. Conclusion: No aromatase activity was detected in the 3D cultured endometrial tissue. Although it is reported that aromatase is expressed in endometrial disease, it is possibly not expressed in this phase in normal endometrium.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".