Genetic variants of rs324420 in fatty acid amide hydrolase gene influence N‐acylethanolamine concentrations in plasma during canola oil multicenter intervention trial (383.7)
Bibliographic record
Abstract
N‐acylethanolamines (NAE) involve in the regulation of energy balance. The genetic polymorphisms of fatty acid amide hydrolase (FAAH) gene, which encodes the NAE degrading enzyme, are associated with obesity. The objective of the current clinical study was to investigate if genetic variants of rs324420 in FAAH gene play a role in regulating circulating NAE levels after five dietary interventions. A double‐blinded randomized crossover clinical trial consisting of five 30‐d periods was conducted. Treatments included 60 g of canola oil (Canola, 59% OA, 20% LA, 10% ALA), high oleic canola oil (CanolaOleic, 72% OA, 15% LA), high oleic canola/DHA oil (CanolaDHA, 63% OA, 13% LA, 6% DHA), corn/safflower oil (CornSaff, 18% OA, 69% LA) and flax/safflower oil (FlaxSaff, 18% OA, 38% LA, 32% ALA). OEA, AEA, PEA, LEA, ALEA, and DHEA plasma levels were measured and genotypes of rs324420 were studied. A total of 130 volunteers completed the study and 129 were successfully genotyped. 85 participants (66%) had CC, and 44 (34%) had CA or AA. Both fatty acid profiles and NAE levels in plasma reflected the dietary fatty acid composition. No differences were observed between two genotypes on OA or DHA levels. However, trends towards higher OEA (p=0.0565) and higher DHEA levels (p=0.0010) were observed in minor allele A carriers across all diets. In the comparison of two OA rich diets, CanolaDHA showed lower OEA level than CanolaOleic feeding (p=0.0006) with no difference in genotypes. CanolaDHA also showed higher DHEA level than CanolaOleic (p<0.0001) and A carriers had higher DHEA level in CanolaDHA (p<0.0001). Our data suggest that rs324420 A carriers favor higher NAE levels because of lowered FAAH activities. But dietary DHA may influence OEA levels due to a preference of such polymorphism for the hydrolysis of DHEA over OEA. The impact of genetic variants of rs324420 may be selective for specific NAEs. Grant Funding Source : Supported by Canola Council of Canada
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".