Interferon‐inducible Protein 6‐16 (IFI‐6‐16, ISG16) promotes Hepatitis C virus replication in vitro
Bibliographic record
Abstract
Interferon-inducible protein 6-16 (IFI-6-16, ISG16) is one of the interferon stimulated genes (ISGs) whose expression levels can be induced by type I interferons. Previous studies from our group indicated that higher expression levels of ISG16 were detected in the pre-treatment liver tissues of patients chronically infected with hepatitis C virus (HCV) who do not respond to interferon-based therapy. However, its role in HCV production is not well defined. This study is to test the effect of over-expression of ISG16 on HCV RNA replication and IFN anti-HCV activity in both HCV replicon cells and JFH-1 HCV culture system (HCVcc). Expression plasmid of ISG16 was constructed and transfected into HCV replicon cells or JFH-1 HCV culture system. After confirmation of the successful expression of ISG16 at mRNA (real-time PCR) and protein (Western Blot) levels, HCV RNA level was analyzed by real-time quantitative PCR in the absence or presence of interferon-α. Over-expression of ISG16 promoted HCV RNA replication in both the Con1b replicon and the HCV JFH-1-based cell culture system and blunted the anti-HCV activity of IFN-α. In conclusion, Over-expression of ISG16 promotes HCV RNA replication and rescues the IFNα-mediated anti-HCV activity.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".