Interferon‐inducible Protein 6‐16 (IFI‐6‐16, ISG16) promotes Hepatitis C virus replication in vitro
Bibliographic record
Abstract
Interferon-inducible protein 6-16 (IFI-6-16, ISG16) is one of the interferon stimulated genes (ISGs) whose expression levels can be induced by type I interferons. Previous studies from our group indicated that higher expression levels of ISG16 were detected in the pre-treatment liver tissues of patients chronically infected with hepatitis C virus (HCV) who do not respond to interferon-based therapy. However, its role in HCV production is not well defined. This study is to test the effect of over-expression of ISG16 on HCV RNA replication and IFN anti-HCV activity in both HCV replicon cells and JFH-1 HCV culture system (HCVcc). Expression plasmid of ISG16 was constructed and transfected into HCV replicon cells or JFH-1 HCV culture system. After confirmation of the successful expression of ISG16 at mRNA (real-time PCR) and protein (Western Blot) levels, HCV RNA level was analyzed by real-time quantitative PCR in the absence or presence of interferon-α. Over-expression of ISG16 promoted HCV RNA replication in both the Con1b replicon and the HCV JFH-1-based cell culture system and blunted the anti-HCV activity of IFN-α. In conclusion, Over-expression of ISG16 promotes HCV RNA replication and rescues the IFNα-mediated anti-HCV activity.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.006 | 0.011 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".