LYCAT, an acyltransferase required for 1‐stearoyl esterification of phosphoinositides, plays a critical role in receptor trafficking dynamics (782.1)
Bibliographic record
Abstract
Phosphoinositides (PtdInsPs) are important signaling lipids that regulate many cellular processes, including membrane trafficking and cell proliferation. There are seven interconvertible species of PtdInsPs based on the inositol headgroup phosphorylation. However, there are additional molecular species of PtdInsPs that differ by their acyl chain type, though the predominant species consists of 1‐stearoyl‐2‐arachidonoyl (18:0/20:4). To date, very little is known about the functional relevance of the various acyl‐dependent PtdInsP species. LYCAT is an acyltransferase that appears responsible for inserting stearoyl at sn‐1 of PtdInsPs. Using siRNA‐dependent gene silencing of LYCAT in cultured cells, we have begun to elucidate the role of LYCAT in membrane trafficking and signaling. We present evidence that LYCAT silencing profoundly alters the trafficking of transferrin and its receptor. Specifically, LYCAT‐silenced cells exhibit reduced transferrin uptake, abated cell surface levels of transferrin receptor and dispersal of the receptor away from a perinuclear distribution. Our data suggest that the above defects are due to a fraction of the transferrin receptor population becoming trapped in a non‐cycling compartment. Thus, LYCAT seems essential for proper receptor sorting and trafficking, likely by controlling the levels of 1‐stearoyl‐containing PtdInsPs. Grant Funding Source : Supported by Canadian Institutes for Health Research
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.005 | 0.004 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".