79 RELATIONSHIPS BETWEEN SURVIVAL RATE AND BIRTH WEIGHT IN CLONED KOREAN NATIVE CALVES
Bibliographic record
Abstract
Cloning of somatic cells has been investigated actively in cattle, but the cloned calves have been characterized by high birth weight and low survival rate. The present study was conducted to investigate the relationships between survival rate and birth weight in cloned and AI calves. The ear skin fibroblasts were obtained from 2- to 3-year-old Korean native cows (Hanwoo) and the cells were cultured in Dulbeccos Modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 38.5°C, 5% CO2 in air. Bovine oocytes collected from ovaries obtained from a nearby slaughterhouse were cultured in vitro and then enucleated, injected with donor cells and fused, and cultured to produce cloned embryos at the blastocyst stage. Somatic cell cloning and in vitro culture of embryos were performed by the procedures described previously (Im et al., 2001 AJAS 14, 759–764, and Im et al., 2001 AJAS 14, 1260–1266). A total of 580 cloned embryos at blastocyst stage were transferred to 293 recipient cows; 32 female calves (5.5%) were born (2 of them were born dead). Thirty-four (15 female and 19 male ) calves (57.6%) were born from 59 artificially inseminated Korean native cows as control. Fifteen of the 32 cloned calves were delivered by caesarean section. However, all the artificially inseminated cows delivered naturally. Birth weights of 30 live cloned calves averaged 31.08 kg (>15 kg: 3, 20 kg: 2, 25 kg: 2, 30 kg: 5, 35 kg: 9, 40 kg: 6, <45 kg: 3), while those of female AI calves averaged 23.67 kg (>15 kg: 0, 20 kg: 3, 25 kg: 6, 30 kg: 6, 35 kg: 0, 40 kg: 0, <45 kg: 0). After calving, 11 of 30 cloned calves survived for more than 365 days (birth weight of these calves averaged 28.25 kg), but 19 of 30 calves died within 175 days and their average birth weight was 32.80 kg (650 kg). Gestation length of cows that received cloned embryos was 287 (279–295) days on average (excluding the data of calves delivered by caesarean section) and that of cows artificially inseminated was 287 (255–293) days. In conclusion, the birth weight was significantly correlated (P < 0.05) with survival rate of cloned calves, and survival rates of calves with extremely high or low birth weights were significantly low. However, there was no relationship between gestation length and survival rate.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".