Sumoylation is crucial for the stability of Leukocyte-specific protein 1 (P5118)
Bibliographic record
Abstract
Abstract Leukocyte-specific protein 1 (LSP1) is an intracellular protein expressed in leukocytes and endothelial cells and regulates several leukocyte and endothelial cell functions including transmigration and chemotaxis of leukocytes in inflammation. There is a discrepancy between its predicted molecular weight (≈37KDa) and that detected in SDS-PAGE (52KDa). To solve this discrepancy, we tested the hypothesis that LSP1 is sumoylated immediately after its translation. Here we demonstrate that, in transfected cells, SUMO1 is conjugated to LSP1 and Ubc9 acts as a SUMO1 ligase for LSP1. Moreover, the sumoylated LSP1 can be desumoylated by the over-expression of SENP1, a SUMO-specific protease. Single lysine to alanine mutation at lysine residue 270 or 318 of recombinant mouse LSP1 prevented its sumoylation in vitro. These LSP1 mutants were degraded rapidly compared to their wild type counterpart. Our results show that sumoylation enhances LSP1 stability, which may ensure its functions in leukocytes and endothelial cells.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".