Role for cholesterol and caveolin‐rich membrane microdomains in controlling the activity Na+/H+ exchange 1 independently of mitogenic stimulation
Bibliographic record
Abstract
The Na + /H + exchanger 1 NHE‐1 is a ubiquitous membrane transporter mainly involved in pH and cell volume regulation. NHE‐1 activation by H + is carried out according to the Monod‐Wyman‐Changeux model in which the dimeric NHE‐1 oscillates between a low and a high affinity conformation, the balance of which being defined by the ratio between the two forms L 0 . Influence of cholesterol‐ and caveolin‐rich microdomains on NHE‐1 activity is examined, by using a cholesterol depleting agent, methyl‐β‐cyclodextrin (MBCD). MBCD induce NHE‐1 activation, by modulating its allosteric balance, these effects being reverted by cholesterol repletion. This activation appears to be associated with NHE‐1 relocalization outside caveolin‐rich microdomains and distinct from mitogenic stimulation of NHE‐1, since MBCD and serum cotreatments are additive; besides, serum alone stimulates this transporter without modifying its localization. Finally, MBCD activate a serum‐insensitive mutated NHE‐1 form and this process occurs independently of MAP kinase phosphorylation. In conclusion, the membrane localization of NHE‐1 in or out microdomains seems to be an essentiel regulator of NHE1 activity, independently of mitogenic action.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".