Molecular characterization and expression patterns of<i>PsSVP</i>genes reveal distinct roles in flower bud abortion and flowering in tree peony (<i>Paeonia suffruticosa</i>)
Bibliographic record
Abstract
Wang, S., Xue, J., Ahmadi, N., Holloway, P., Zhu, F., Ren, X. and Zhang, X. 2014. Molecular characterization and expression patterns of PsSVP genes reveal distinct roles in flower bud abortion and flowering in tree peony (Paeonia suffruticosa). Can. J. Plant Sci. 94: 1181–1193. Container culture and flower forcing are used for off-season production of tree peony for the Chinese Spring Festival. Storage of potted tree peony for 10 d at 12°C in a refrigerator before 4°C chilling treatment can help new root growth and promote leaf development. Development from bud swelling to anthesis was divided into nine stages. Some aborted flower buds usually emerge in Stage III. Removal of two to four leaflets in an alternating pattern and applying gibberellic acid 3 (GA3) around the flower bud at Stage III can decrease the flower bud abortion rate and promote flower formation rate. Two MADS-box genes with homology to Arabidopsis SVP, designated PsSVP1 and PsSVP2, which probably caused flower-bud abortion, were isolated by reverse transcription-PCR. Sequence comparison analysis showed that PsSVP was most similar to SVP-like gene in apple. Phylogenetic analysis indicates that PsSVP was evolutionarily close to SVP-like genes from Malus domestica, SVP genes from Cruciferae and SVP-like genes from Vitis vinifera. The qRT-PCR results suggested that expression of PsSVP was high in vegetative growth phase, especially in the leaves of tree peony, and its expression was regulated by GA3. Further analysis showed that more PsSVP transcripted in the aborted flower bud, especially in the buds where leaflets grew well. It was deduced that PsSVP can promote vegetative growth and suppress flowering in tree peony. Thus, it is very important to further investigate PsSVP and decipher the mechanisms of flower-bud abortion to improve forcing culture of tree peony.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".