Superoxide anion mediates Ang II‐induced ET‐1 expression in mouse adventitial fibroblasts
Bibliographic record
Abstract
Our objective was to determine if superoxide anion mediates endothelin‐1 (ET‐1) expression in adventitial fibroblasts in response to angiotensin II (Ang II). Vascular adventitial fibroblasts were isolated from mouse aorta, preproET‐1 messenger RNA (mRNA) levels were determined by relative RT‐PCR and procollagen protein was measured by Western blot. Adventitial fibroblasts were treated with or without Ang II (10 −7 M) and antioxidants superoxide dismutase (SOD) (350 U/ml), tempol (10 −5 M), and tiron (10 −5 M). Ang II significantly increased preproET‐1 mRNA and procollagen expression while treatment with antioxidants decreased both significantly (p<0.05). To further examine the role of superoxide, Cu 2+ /Zn 2+ SOD was overexpressed in the adventitial fibroblasts and decreased levels of preproET‐1 mRNA and procollagen were found with Ang II treatment when compared to control (p<0.05). In conclusion, Ang II is able to increase both superoxide and ET‐1 production and inhibition of superoxide leads to a decrease in ET‐1 expression demonstrating that superoxide mediates Ang II induced ET‐1 expression in mice adventitial fibroblasts. Funding provided by the Canadian Institute of Health Research.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".