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Record W1789680410

A Method to Identify DNA Sequences Contained in an Arbitrary Nuclear Region

2011· dissertation· en· W1789680410 on OpenAlexvenueno aff
David Anchel

Bibliographic record

VenueLibrary and Archives Canada (Government of Canada) · 2011
Typedissertation
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicBiotin and Related Studies
Canadian institutionsnot available
Fundersnot available
KeywordsLocus (genetics)DNABiologyNuclear localization sequenceComputational biologyGeneNuclear proteinGeneticsCell nucleusImmunoprecipitationNuclear DNAIdentification (biology)PhysicsMolecular biologyTranscription factor
DOInot available

Abstract

fetched live from OpenAlex

Nuclear substructures known as “nuclear bodies” associate with particular gene loci, and this may determine or reflect a mechanism of genetic control. The detection of these associations currently relies on the use of loci-specific probes, or immunoprecipitation of bulk cells with a particular protein. However, there is evidence that these associations respect the nuclear body, not necessarily any one constituent protein, and are statistically and/or functionally significant even when they occur over distances resolvable by light microscopy, or within single cells. A method is proposed that will allow for the identification of loci contained within the vicinity of an arbitrary nuclear structure in a single cell. It is demonstrated that the crucial aspects of this method are feasible; that DNA sequences originating from arbitrary subnuclear regions targeted by two-photon irradiation can be determined, and identified to a particular locus.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.149
Threshold uncertainty score0.901

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.206
Teacher spread0.200 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2011
Admission routes1
Has abstractyes

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