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Record W1791047623 · doi:10.1002/9780470027318.a1427

Structural Analysis of Ribozymes

2000· other· en· W1791047623 on OpenAlexaff
Sirinart Ananvoranich, Jean‐Pierre Perreault

Bibliographic record

VenueEncyclopedia of Analytical Chemistry · 2000
Typeother
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRNA and protein synthesis mechanisms
Canadian institutionsUniversité de Sherbrooke
Fundersnot available
KeywordsRibozymeLigase ribozymeComputational biologyHairpin ribozymeRNAMammalian CPEB3 ribozymeBiologyStructural motifHEPATITIS DELTANucleic acid structureGenomeGeneticsChemistryBiochemistryGene

Abstract

fetched live from OpenAlex

Abstract We use delta ribozymes as a model in illustrating the various steps involved in the structural analysis of ribozymes. Delta ribozymes were originally identified as a self‐cleaving motif located on the single‐stranded RNA genome of hepatitis delta virus (HDV). The methodologies are presented in the order of their requirement for determination of the structure/function relationship of a ribozyme, beginning with the preparation of the RNA molecules required for the studies. Primary structural information is needed in order to identify potential ribozymes, then secondary and tertiary structural information is required for ribozyme characterization. The manuscript was written to guide an investigator from the initial observation of RNA catalytic activity (a ribozyme discovery) to the deduction of a structural model of the ribozyme.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Insufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: Not applicable
GenreCandidate signal: Other · Consensus signal: Other
Teacher disagreement score0.108
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.000
Insufficient payload (model declined to judge)0.0100.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.240
Teacher spread0.234 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designNot applicable
Domainnot available
GenreOther

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2000
Admission routes1
Has abstractyes

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