The Mn‐binding site and newly identified C‐terminal metal binding site both play important roles in the thermal stability of Mn‐peroxidase.
Bibliographic record
Abstract
Extracellular fungal peroxidases such manganese peroxidase (MnP) offer potential in biomass utilization, biomaterial manufacture and detoxification of recalcitrant pollutants. Use of these enzymes in industrial processing requires a detailed understanding of their thermal tolerance limits. Although the role of calcium in protecting MnP from thermal inactivation has been studied extensively, the roles of the Mn‐binding site and C‐terminus in the thermal stability of MnP remain unexplored. In this work, we examine the effects of exogenous metals on the residual activities, heme Soret absorptions, and secondary structures of MnP. Analysis of the wild‐type MnP indicates that, at low pH, addition of exogenous Mn2+ stabilizes the protein to a greater extent than Ca2+. Analyses of the Mn‐binding site variants, D179N and E39D, indicate that the stabilization is due to interactions at the Mn‐binding site. A new variant, D84N, at the newly identified C‐terminal metal binding site is also examined. The data indicate that this secondary site also contributes to thermal stability, although to a lesser extent than mutations at the Mn‐binding or Ca‐binding sites.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".