Bibliographic record
Abstract
MUC2 in the major secreted gel‐forming mucin produced by goblet cells (GC) that form the protective mucus blanket covering the epithelium as the first line of innate host defense in the gut. The protein complex and the cellular mechanisms by which MUC2 granulae are released by compound exocytosis from GC under basal or in response to mucus secretagogues are not known. In this study we identified the putative exocytosis components expressed in human GC, LS 174T. The exocytosis protein complex formation between SNAP23, Syntaxin 3 and Munc18b was studied in response to the PKC agonist, phorbol 12‐myristate 13‐acetate (PMA) using immunoprecipitation and mass spectrometry. In contrast to MUC2, the exocytosis proteins were not regulated at the transcriptional level, as mRNA and protein expression was unaltered after PMA. However, PMA treatment phosphorylated Syntaxin 3 in active SNARE complex formation. Surprisingly, confocal imaging revealed that Syntaxin 3 and Munc18b are not only localized to the apical cap of the GC theca but also to areas around the theca where the SNARE components co‐localized with actin filaments. These findings were corroborated using differential centrifugation where Syntaxin 3 was identified on both the granulae and the plasma membrane. Our studies reveal complex exocytosis protein machinery that regulates both basal and stimulated MUC2 granulae release from goblet cells. Supported by CIHR.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".