238. CCN1 Expression By Fibroblasts is Required for Bleomycin-Induced Skin Scleroderma
Bibliographic record
Abstract
Background: The cellular microenvironment is being increasingly appreciated as playing a key role in CTDs including scleroderma. Matricellular proteins, such as members of the CCN family (CCN1–6) play a key role in local modifications of cellular signalling and are dysregulated in fibrotic conditions including scleroderma. CCN2 (CTGF) has been shown to play a key role in skin biology and fibrosis; however, the role of CCN1 (cyr61) in these processes are unclear. Methods: We use fibroblast-specific CCN1 knockout mice (employing floxed CCN1 mice and a tamoxifen-dependent Cre recombinase expressed under the control of a fibroblast-specific collagen 1A2 promoter) and the bleomycin model of skin scleroderma to assess the potential role of CCN1 to skin connective tissue biology and fibrosis. Fibrosis is evaluated histologically using haematoxylin and eosin and trichrome stains, and molecularly using a collagen ELISA to detect insoluble and soluble collagen levels and real-time PCR analysis to assess messenger RNA (mRNA) expression.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.005 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".