INORGANIC CARBON REPLETION DISRUPTS PHOTOSYNTHETIC ACCLIMATION TO LOW TEMPERATURE IN THE CYANOBACTERIUM <i>SYNECHOCOCCUS ELONGATUS</i><sup>1</sup>
Bibliographic record
Abstract
Acclimation of cyanobacteria to ambient fluctuations in inorganic carbon (Ci) and temperature requires reorganization of the major protein complexes involved in photosynthesis. We grew cultures of the picoplanktonic cyanobacterium Synechococcus elongatus Naegeli across most of its range of tolerable temperatures from 23 to 35°C at both low (<0.1 mM) and high Ci (approximately 4 mM). Over that range of temperatures, the chl‐based doubling time did not differ between low and high Ci grown cells but did increase with decreasing temperature. Cells grown at 23°C high Ci showed an elongated morphology, which was not present in 23°C low Ci cells nor at 35°C high and low Ci. Furthermore, 23°C high Ci cells showed premature senescence and death compared with all other treatments. Phycocyanin per cell was greater in high Ci grown cells at all temperatures but showed a characteristic decrease with decreasing temperature. Functional PSII determination showed that 23°C high Ci cells had 1.5 × 105 PSII·cell–1 compared with only 6.9 × 104 PSII·cell–1 for 23°C low Ci. The 35°C high and low Ci cells had 7.7 × 104 and 6.4 × 104 PSII·cell–1, respectively. These data were supported by immunoblot determinations of PsbA content·cell–1. As a result of their high PSII·cell–1, 23°C high Ci cells generated more reductant from PSII than could be accommodated by downstream assimilative metabolism, resulting in early senescence and death of 23°C high Ci cells, probably as a result of the generation of reactive byproducts of electron transport.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".