Sperm Y:X chromosome ratio and androgen response in men exposed to environmental pollutants
Bibliographic record
Abstract
The last several decades have seen an increase in male infertility concomitant with a decline in the birth sex ratio. The conditions are believed to be linked to increased exposure to endocrine disrupting chemicals (EDCs), including the persistent organic pollutants or lifestyle factors e.g. smoking. The mechanism behind the lowered birth sex ratio is unknown, but might be due to an EDC-induced skewing of the proportions of Y to X chromosome bearing sperms. The aims of this thesis were: to elucidate possible associations between the sperm Y:X ratio and EDCs in several highly exposed populations, to evaluate any relationships that might exist between the Y:X ratio and sperm parameters in subfertile men, and to investigate the effect of smoking on androgen regulation in vivo as well as in vitro. The sperm sex chromosome ratio was investigated by fluorescent in situ hybridization (FISH) in n=449 men from the Faroe Islands and compared to n=414 men from Greenland, Ukraine and Poland. Infertile men n=190 were enrolled at a fertility clinic in Denmark and androgenicity in smoking men was measured in n=2156 men recruited for several previous epidemiological studies. The effect of smoking was assessed in a reporter system with the prostate specific antigen promoter driving a luciferase gene, in the presence or absence of various concentrations of the cigarette smoke constituent Benzo[α]Pyrene (BaP). In men from the Faroe Islands, the Y:X ratio was lower than in other highly exposed populations and a negative association between the sperm sex chromosome ratio and both dichlorodiphenyl dichloroethane (DDE) and selected polychlorinated biphenyls (PCBs) in the somewhat older, proven fertile men was found. A positive trend between the Y:X ratio and perfuorooctane sulfonate (PFOS) in a combined population of men from Greenland, Ukraine and Poland was found. The opposite association was observed in the Greenland Inuit alone. In subfertile men, a positive association between the Y:X ratio and progressive motility was noted. In two cohorts of smoking men, positive trends between groups of men with increasing exposure to cigarette smoke and androgen insensitivity was found. The in vitro experiments showed a significant deactivation of the AR at the highest BaP concentration. To conclude, high concentrations of EDCs influence the Y:X ratio in highly exposed populations i.e. men from Greenland and the Faroe Islands. The inter-population differences in Y:X ratio might be genetic or due to lifestyle factors such as diet. Men with low progressive motility had lower Y:X ratio, possibly explaining the low birth sex ratio seen following intra cytoplasmic sperm injection. Indications that smoking has adverse effects on the androgenicity were observed both in vivo and in vitro.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.002 | 0.002 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".