25‐Hydroxycholesterol inhibits adipogenic differentiation of C3H10T1/2 mouse stem cells through inhibition of PPARγ expression (1094.2)
Bibliographic record
Abstract
Oxysterols have been shown to inhibit adipogenic differentiation of mesenchymal stem cells while inducing their osteogenic differentiation. However, the effects of oxysterols on the adipogenic differentiation of C3H10T1/2 mouse stem cells are poorly understood. This study was conducted to examine the effects of specific oxysterols on the expression of adipogenic transcripts and adipogenic differentiation of C3H10T1/2 mouse stem cells. Cells were treated with 10% Fetal Bovine Serum (control), 500nM Dexamethasone, 0.5mM 3‐Isobutyl‐1‐methlyxanthine, 20µg/ml Insulin (DMI) + 10µM PPARγ agonist Troglitazone (Tro) (DMITro) and DMITro + 10µM 20S hydroxycholesterol (20S), 25 hydroxycholesterol (25), 22R hydroxycholesterol (22R) or 22S hydroxycholesterol (22S) for six days. Gene expression was analysed by real‐time quantitative polymerase chain reaction and expression data analysed using the Statistical Analysis Software (SAS 9.2). Compared to the control, DMITro significantly increased the expression of peroxisome proliferator‐activated receptor γ (PPARγ), CCAAT/enhancer binding protein α (C/EBPα), fatty acid binding protein 2 (FABP2) and lipoprotein lipase (LPL). Compared to DMITro, treatment of cells with DMITro + 25 significantly decreased the expression of PPARγ, the master regulator of adipogenesis (P 蠄 0.05). Expression of C/EBPα was significantly decreased by DMITro + 25 and DMITro + 20S (P 蠄 0.05), while the expression of FABP2 was significantly decreased by DMITro + 25, DMITro + 20S and DMITro + 22R (P 蠄 0.05). Lastly, the expression of LPL was significantly decreased by DMITro + 25 and DMITro + 22R (P 蠄 0.05). In conclusion, the results showed that 25 hydroxycholesterol was the most potent in inhibiting the expression of key adipogenic transcripts and adipogenesis of C3H10T1/2 mouse stem cells suggesting its potential application in reducing adipogenesis and obesity. Key words: Oxysterols, C3H10T1/2 stem cells, differentiation, PPARγ Grant Funding Source : Supported by Canadian natural science and engineering research council.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".