Improved production of the indole alkaloid canthin-6-one from cell suspension culture of Brucea javanica (L.) Merr
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Bibliographic record
Abstract
In an attempt to increase the productivity of the pharmaceutical compound canthin-6-one from cell suspension culture of Brucea javanica, a high cell density culture and improved culture conditions have been investigated with focus on culture conditions and stages of growth. The highest yield of canthin-6-one, 26.72 mg g -l dry cell, was produced intracellular in 50 g L -1 cell mass. The established cell suspensions were harvested at the age of 40 days and placed into MS medium containing 1.0 mg L-1 2,4-D, 1.0 mg L-1 NAA, 0.1 mg L-1 Kinetin and 10 mg L -1 tryptophan for 33-40 days, approximately when the cultures were at their late stationary phase of growth. Addition of 20 mg L -1 tryptophan did not show a significant difference in canthin-6-one production (p>0.05). Higher than 10 mg L -1 of tryptophan showed adverse effect on the concentration of canthin-6one alkaloid (p=0.01). Qualitative analysis of chloroform-extracts of dried cells from suspension cultures revealed spots on TLC; and the identity of canthin-6-one was established by comparison of its R f value (R f =0.37), its colour reaction with Dragendorff reagent and ammonium sulphate and its behaviour in UV with an authentic sample. Quantification of canthin-6-one alkaloid using TLCdensitometry scanner in response to different concentration of tryptophan as a precursor showed a concentration-dependent response. The production of canthin-6-one at 26.72 mg g -1 dried cell weight in this study, establishes a methodology of an improved procedure compared to those previously reported and offers an opportunity for production of canthin-6-one alkaloid at an industrial level.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it