Improved production of the indole alkaloid canthin-6-one from cell suspension culture of Brucea javanica (L.) Merr
Bibliographic record
Abstract
In an attempt to increase the productivity of the pharmaceutical compound canthin-6-one from cell suspension culture of Brucea javanica, a high cell density culture and improved culture conditions have been investigated with focus on culture conditions and stages of growth. The highest yield of canthin-6-one, 26.72 mg g-l dry cell, was produced intracellular in 50 g L-1 cell mass. The established cell suspensions were harvested at the age of 40 days and placed into MS medium containing 1.0 mg L-1 2,4-D, 1.0 mg L-1 NAA, 0.1 mg L-1 Kinetin and 10 mg L-1 tryptophan for 33-40 days, approximately when the cultures were at their late stationary phase of growth. Addition of 20 mg L-1 tryptophan did not show a significant difference in canthin-6-one production (p>0.05). Higher than 10 mg L-1 of tryptophan showed adverse effect on the concentration of canthin-6- one alkaloid (p=0.01). Qualitative analysis of chloroform-extracts of dried cells from suspension cultures revealed spots on TLC; and the identity of canthin-6-one was established by comparison of its Rf value (Rf=0.37), its colour reaction with Dragendorff reagent and ammonium sulphate and its behaviour in UV with an authentic sample. Quantification of canthin-6-one alkaloid using TLCdensitometry scanner in response to different concentration of tryptophan as a precursor showed a concentration-dependent response. The production of canthin-6-one at 26.72 mg g-1 dried cell weight in this study, establishes a methodology of an improved procedure compared to those previously reported and offers an opportunity for production of canthin-6-one alkaloid at an industrial level. Keywords: Canthin-6-One, cell suspension, Brucea javanica, secondary metabolites
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".