Intrinsic phenotypic differences of the asthmatic epithelium and its inflammatory responses to RSV and particulate matter
Bibliographic record
Abstract
Within the airway, the epithelium forms the first structural cell defense against common environmental insults such as respiratory syncytial virus (RSV) and particulate matter. These stimuli have been shown to be associated with exacerbations of the disease, which contribute to the financial burden of asthma. Objective: Characterize the intrinsic phenotype of asthmatic and non-asthmatic airway epithelium and determine their inflammatory responses to RSV and particulate matter (EHC-93) exposure. Methods: Air-liquid interface cultures (ALICs) were generated from asthmatic and non-asthmatic airway epithelial cells. Airway tissue and ALICs were analyzed by immuno for Cytokeratin-5, E-cadherin, Ki67, Muc5AC, and apoptosis. ALI cultures were exposed to RSV (4×10 6 PFU/ml) or EHC-93 (100μg/ml) for 24, 48, and 96 h and supernatants analyzed for pro-inflammatory cytokines using ELISA. Results: The airway epithelium of asthmatics in vivo and in ALIC demonstrated a less differentiated epithelium characterized by elevated numbers of cells expressing basal cell markers CK-5 and Ki67 and less adherens junction protein E-cadherin (p Conclusion: This parallel ex vivo and in vitro study demonstrates that the asthmatic epithelium displays an intrinsic alteration in phenotype and responds with an enhanced inflammatory profile to RSV and particulate matter.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".