Genetic Transformation of <i>Brassica napus</i> with <i>MSI-99m</i> Gene Increases Resistance in Transgenic Plants to <i>Sclerotinia sclerotiorum</i>
Bibliographic record
Abstract
Magainins are a class of antimicrobial peptides isolated from skin secretions of the African clawed frog Xenopus laevis . MSI-99 is a synthesized magainin II analogue with inhibitory effects to a wide spectrum of microbial organisms, including bacteria, fungus and viruses. Sclerotinia sclerotiorum is one of the most destructive pathogens in rape ( Brassica napus ), causing devastating yield losses. To evaluate its resistance to rape Sclerotina rot, we transferred the MSI-99m gene (modified MSI-99 ) into Chinese rape variety Zhongyou 821 using Agrobacterium -mediated method. Nine transformed lines carrying a MSI-99m expression vector were detected by polymerase chain reaction (PCR), among which seven lines expressed MSI-99m gene according to qRT–PCR analysis. Disease resistance analysis consistently showed that the high level expression of MSI-99m increased resistance to S. sclerotiorum in transgenic rape lines. This result demonstrated that MSI-99m gene may be applied as a resistant gene resource in rape for the improvement of rape varieties.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".