Dielectric properties of CHO cells obtained using a microwave interferometer based dielectrophoresis cytometer
Bibliographic record
Abstract
Studying biological phenomena at the cellular level allows understanding the basics of cellular function and the mechanism of their interactions with internal or external stimuli. Among available single cell analysis techniques such as, fluorescent labeling, magnetic labeling, mechanical, and electrical approaches, electrical ones are of special interest due to their minimum influence on the natural state of cells. Dielectrophoresis (DEP) cytometry- translation of cells in a non-uniform electric field- is an electrical based technique previously employed for differentiation and characterization of cells (R. Pethig, Biomicrofluidics, 2010). In this work we employ a microwave based DEP cytometry technique that we have developed to obtain dielectric parameters of Chinese Hamster Ovary (CHO) cells, a cell line with prominent pharmaceutical applications for production of therapeutic proteins. Cell electrical parameters are not well established. We present a model for CHO using a double shell model comprising the cell membrane, cytoplasm, nuclear envelope, and nucleus.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".