Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that suppresses cell elongation
Bibliographic record
Abstract
Transcription factors regulate multiple aspects of plant growth and development. Here we report the identification and functional analysis of a plant-specific, novel transcription factor in Arabidopsis. We isolated a dominant, gain-of-function mutant that displays reduced lengths in all aerial organs including hypocotyl, rosette leaf, cauline leaf, inflorescence stem, floral organs and silique. Molecular cloning revealed that these phenotypes are caused by elevated expression of the Arabidopsis thaliana Ovate Family Protein 1 (AtOFP1). This mutant was designated as Atofp1-1D. We show that the altered morphology of Atofp1-1D mutant is caused by reduced cell length resulting from reduced cell elongation, and demonstrate that a mutant harboring a transposon insertion that disrupts the OVATE domain of AtOFP1 is indistinguishable from wild-type plants. Plants overexpressing other closely related AtOFP genes phenocopy plants overexpressing AtOFP1, implying a possible overlapping function among members of the AtOFP gene family. We found that AtOFP1 localizes in the nucleus, and that AtOFP1 functions as an active transcriptional repressor. Chromatin immunoprecipitation results indicated that AtGA20ox1, a gene encoding the key enzyme in GA biosynthesis, is a target gene regulated by AtOFP1. Consistent with this, exogenous gibberellic acid can partially restore defects in cell elongation in plants overexpressing AtOFP1, suggesting that such a reduced cell elongation is caused, in part, by the deficiency in gibberellin biosynthesis. Taken together, our results indicate that AtOFP1 is an active transcriptional repressor that has a role in regulating cell elongation in plants.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".