Genetic and Expression Analyses of a Male Sterile Gene <i>MGA1(t)</i> in Rice (<i>Oryza sativa</i> L. )
Bibliographic record
Abstract
A male gamete sterile mutant (tentatively named Male gametophyte abortion1 , mga1 ( t )) was obtained by abnormality screening of transgenic rice materials in this study. The ratio of marker gene segregation was 1:1 by χ 2 - test and the pollen grains of the mutant were half sterile. The phenotype of pollen abortion was found co-segregating with T-DNA insertion in mga1 ( t ) mutant, and T-DNA passed only through the female gamete. Southern blot analysis indicated that T-DNA inserted into the genome of the mutant was single copy. Flanking sequence analysis showed that T-DNA was inserted into the 5' untranslated region of a Bax inhibitor (BI) - 1 like family protein gene in rice chromosome 3. BI - 1 like protein gene was predicted to be involved in programmed cell death. RT-PCR expression analyses revealed that MGA1 ( t ) gene expressed during the whole growth and development stages of rice, and had a strong expression pattern in the spikelets with the length about 0~7 mm. These results indicated that MGA1 ( t ) gene was a male-preferential expressed gene.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".