Development of Insect Resistant Transgenic Lines Mediated by <i>Agrobacterium</i> in Cotton (<i>Gossypium hirsutum</i> L.)
Bibliographic record
Abstract
In order to produce transgenic cotton resistant to insects, five months old embryogenic calli of Coker 310 were transformed with Agrobacterium tumefaciens strain LBA4404 harbouring the plasmid pBI 121 containing cry2Ab gene under the control of CaMv 35S promoter. Neomycin phosphotransferase (nptII) gene was used as a selectable marker. Infected embryogenic calli were incubated in dark at 24°C in co-cultivation medium containing 100mM acetosyringone for three days. Transformed calli were selected on selection medium containing 25 mg/L kanamycin and 500 mg/L cefotaxime. After four rounds of selection (each round with 15 days duration) the induction of kanamycin resistant embryogenic calli was at an average frequency of 37.0% and further two subcultures (each with one month duration) of the kanamycin resistant embryogenic calli in the same media resulted in the formation of somatic embryos at an average frequency of 23.7%. The matured cotyledonary stage embryos were germinated on the plantlet development medium without kanamycin selection. Out of 500 explants co-cultivated, 5 plants (1.0% transformation efficiency) showed the presence of genes nptII and cry2Ab through PCR analysis. Insect bioassay against Helicoverpa armigera performed with the five T 0 plants showed 33.0% to 67.0% larval mortality.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".