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Characterization of cuticular chitin‐binding proteins of <i>Leptinotarsa decemlineata</i> (Say) and post‐ecdysial transcript levels at different developmental stages

2010· article· en· W1957863999 on OpenAlex
J. Zhang, Yvan Pelletier

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueInsect Molecular Biology · 2010
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicStudies on Chitinases and Chitosanases
Canadian institutionsAgriculture and Agri-Food Canada
Fundersnot available
KeywordsLeptinotarsaBiologyPhylogenetic treeConsensus sequenceCuticle (hair)Multiple sequence alignmentChitinTriad (sociology)InsectStructural motifSequence alignmentGeneticsPeptide sequenceBiochemistryGeneBotanyLarva

Abstract

fetched live from OpenAlex

Seven cuticle chitin-binding proteins (Ld-CP1v1 to 7) were deduced from antenna cDNAs of adult Colorado potato beetles, Leptinotarsa decemlineata (Say), based on their consensus sequences. The mature proteins consisted of 87-188 residues. Ld-CP1v1 formed a distinct orthologous protein cluster (OP1) along with four proteins from other insect species in a neighbor-joining phylogenetic tree. These proteins also contained a proline glutamine-rich (PQ-rich) region and a highly conserved C-terminal motif (Phr). Their consensus region lacked the defined aromatic triad. Ld-CP2 to 6 clustered with those bearing RR-1 consensus and Ld-CP7 with RR-2 consensus. Ld-CP1v1 to 4 were expressed at the post-ecdysial period in all the developmental stages whereas Ld-CP5 to 7 were expressed mainly in adults.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.046
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.236
Teacher spread0.226 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it