Presence of a Bioactive and Immunoreactive Growth-Hormone-Releasing-Factor-Like Substance in Porcine Placenta
Bibliographic record
Abstract
The present study was conducted to establish whether growth hormone (GH) releasing factor (GRF) is present in porcine placenta. Various concentrations of placental extracts from sows at 90 and 110 days of gestation were added to porcine anterior pituitary cells in culture. The extract-induced GH secretion was compared to that of GRF(1-29)NH2. Experiments were also performed in the presence of the GRF receptor antagonist N alpha-acetyl(D-Arg2-Ala15) rat GRF(1-29)NH2 (10(-7) M). Significant effects on GH production were obtained with increasing concentrations of placental extracts (p < 0.001). This response was significantly diminished in the presence of the GRF receptor antagonist (p < 0.001). The effect of gestational age at the time of collection of the placenta was nonsignificant (p > 0.1). In addition, using a placental extract from 110 days of gestation, an immunoreactive GRF content of 52-63 ng/mg dry weight was detected. Altogether, the detection of an immunoreactive GRF-like substance in porcine placental extracts and the ability of these extracts to stimulate GH release in a concentration-dependent manner, likely by triggering a GRF-receptor-mediated mechanism, support the presence of GRF in this tissue.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".