Pulp and Paper Mill Effluents Contain Neuroactive Substances That Potentially Disrupt Neuroendocrine Control of Fish Reproduction
Bibliographic record
Abstract
Here we show for the first time that components of pulp and paper mill effluents contain neuroactive substances that may impair fish reproduction. Grab samples of primary and secondary effluent were obtained from a representative pulp and paper mill in Eastern Canada. Effluents were fractionated using classic polarity and polyphenolic extraction methods into solvents of selected polarities (water, ethanol, ethyl acetate, and hexane). By means of in vitro, competitive assays on goldfish (Carassius auratus) brain tissues, the extracts were screened for their ability to interact with enzymes and receptors involved in gamma-aminobutyric acid (GABA), dopamine, glutamate, and acetylcholine-dependent neurotransmission. These neurotransmission pathways have essential regulatory roles in fish reproduction. Radioligand binding to the following neurotransmitter receptors were significantly impacted following in vitro incubations with extracts (percentage change from controls indicated in brackets): dopamine-2 (D2; 21-48% increase), GABA(A) receptor binding (65-67% decrease and 189% increase), N-methyl-o-aspartic acid (NMDA; 26-75% decrease), and muscarinic cholinergic (mACh; 42% increase). Activities of the following neurotransmitter-related enzymeswere significantly impacted: monoamine oxidase (MAO; 14-48% decrease), GABA-transaminase activity (33% decrease and 21-69% increase), and acetylcholinesterase (AChE; 21-50% decrease). No changes in glutamic acid decarboxylase (GAD) activity were detected. These findings provide a novel and plausible mechanism by which pulp and paper mills effluents impair fish reproduction by interacting with neurotransmitter systems. Furtherwork is required to identify the active compounds and explore whether these changes occur in vivo.
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How this classification was reachedexpand
Direct model labels (unvalidated)
Per-model category and study-design labels from the labeling rounds. They are machine output, unvalidated, and the disagreement between models ships as data. No study design here is MEDLINE-validated yet.
| Model arm | Categories | Study design | Confidence |
|---|---|---|---|
| gemma | no category Domain: not available · Genre: Empirical About the Canadian research system: no · About a Canadian topic: no | Observational | low |
| gpt | no category Domain: not available · Genre: Empirical About the Canadian research system: no · About a Canadian topic: no | Bench or experimental | high |
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedLabeled directly by 2 models reading the full record.
The models disagree on parts of this classification; every voice is preserved in the section at the end of the page.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".