Regulation of inorganic carbon acquisition by phosphorus limitation in the green alga<i>Chlorella emersonii</i>
Bibliographic record
Abstract
Inorganic phosphate (P i ) plays a central role in cellular energy transduction. As a consequence, limitation of growth by phosphate availability can have an important impact on various aspects of metabolism. Since carbon acquisition via CO 2 -concentrating mechanisms (CCMs) in most microalgae is an active process, requiring ATP, it might be expected that phosphate limitation could have an indirect regulatory influence on CCM activity. We grew the green alga Chlorella emersonii Shihira et Krauss in semicontinuous or continuous cultures in nutrient-replete conditions or with orthophosphate as the limiting nutrient. CCM activity was down-regulated by P limitation. K 0.5 (dissolved inorganic carbon) values increased from approximately 4.5 µmol·L 1 in cells growing at close to maximal rates to >12 µmol·L 1 in cells growing at 0.2 d 1 . Maximal rates of photosynthesis decreased by approximately half over the same range of growth rates. Direct measurements of CCM activity showed that internal CO 2 : external CO 2 ratio was markedly decreased under P limitation, and concurrent measurements of stable carbon isotope discrimination were consistent with decreased CCM activity in the P-limited cells.Key words: phosphate, CCM, CO 2 -concentrating mechanism, Chlorella, inorganic carbon acquisition.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".