Identification of wheat-<i>Thinopyrum intermedium</i> alien disomic addition lines conferring resistance to stripe rust
Bibliographic record
Abstract
Thinopyrum intermedium carries many useful traits for wheat genetic improvement. To identify genes conferring resistance to stripe rust caused by Puccinia striiformis f. sp. tritici in Zhong4, one of the Zhong series of partial amphiploids from the hybrids of common wheat × Th. intermedium, a cross was made between Zhong4 and a Chinese Spring ph1b mutant (CS-ph1b). The parents, F1, F2 plants and F5 lines of the cross were tested with Chinese predominant race CYR31 of P. striiformis f. sp. tritici in the seedling stage under controlled greenhouse conditions. Of 201 F2 seedlings tested with race CYR31, 149 were resistant and 52 were susceptible. The segregation fit a 3 resistant:1 susceptible ratio, indicating that a single dominant gene conferred resistance to the race CYR31. This result was further confirmed by the segregation of F5 lines into 1:1 ratio (P = 0.77). Using the Schiff dyeing method, the chromosome number in the root-tip cells of 10 F5 resistant lines (total 42 plants) was determined. Two alien addition lines (5-1-2-1-1-5, 5-1-2-1-1-6) with the smallest chromosome number (2n = 42 + II) were identified. A total of 105 pollen mother cells were examined at metaphase 1 (PMCs MI) for meiotic chromosome pairing. The lines 5-1-2-1-1-5 and 5-1-2-1-1-6 showed regular meiosis, exhibiting 22 ring or rod bivalents (2n = 22 II). The GISH results indicated that lines 5-1-2-1-1-5 and 5-1-2-1-1-6 were wheat-Th. intermedium alien disomic addition lines with 21 pairs of wheat chromosomes and one pair of Th. intermedium chromosomes. These two lines were all resistant to stripe rust, suggesting that they can be used as donors of stripe rust resistance genes and have great potential to play important roles in wheat stripe rust resistance breeding programs in China and the world.Key words: Wheat-Thinopyrum intermedium, stripe rust, GISH, cytological identification
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".