Characterization of 3-Ketosteroid 9α-Hydroxylase, a Rieske Oxygenase in the Cholesterol Degradation Pathway of Mycobacterium tuberculosis

KshAB (3-Ketosteroid 9α-hydroxylase) is a two-component Rieske oxygenase (RO) in the cholesterol catabolic pathway of Mycobacterium tuberculosis. Although the enzyme has been implicated in pathogenesis, it has largely been characterized by bioinformatics and molecular genetics. Purified KshB, the reductase component, was a monomeric protein containing a plant-type [2Fe-2S] cluster and FAD. KshA, the oxygenase, was a homotrimer containing a Rieske [2Fe-2S] cluster and mononuclear ferrous iron. Of two KshAB the of was the of the of KshAB was in the of a of of KshA, and a of of the in characterized a and of is by a in the the in the and the the is a of a enzyme of and KshAB (3-Ketosteroid 9α-hydroxylase) is a two-component Rieske oxygenase (RO) in the cholesterol catabolic pathway of Mycobacterium tuberculosis. Although the enzyme has been implicated in pathogenesis, it has largely been characterized by bioinformatics and molecular genetics. Purified KshB, the reductase component, was a monomeric protein containing a plant-type [2Fe-2S] cluster and FAD. KshA, the oxygenase, was a homotrimer containing a Rieske [2Fe-2S] cluster and mononuclear ferrous iron. Of two KshAB the of was the of the of KshAB was in the of a of of KshA, and a of of the in characterized a and of is by a in the the in the and the the is a of a enzyme of and Mycobacterium the of the and has a in the of in the a of the of the of is cholesterol catabolic pathway of in cholesterol and in cholesterol is the of the it and of the in the of cholesterol and the pathway the of cholesterol in cholesterol catabolic pathway of of the and the in a it is the of is a of the is by in the of of the of and KshAB 9α-hydroxylase) the of of is a and the the of and is in tuberculosis. the of cholesterol in pathogenesis, a of the of the in the of is a oxygenase (RO) Rieske of of of of of ferrous a reductase and oxygenase a plant-type cluster and a a by two and two and a mononuclear by two and the in in and Mycobacterium KshAB the of and and the of KshAB is is the of the by enzyme and a of in of in a and two and the reductase the oxygenase, a characterized by the of by been in and the oxygenase two it is the the [2Fe-2S] cluster and a the mononuclear two the the the [2Fe-2S] cluster and the mononuclear of of and the of the the two and a in the of in the of in the of and of has been in the of characterized the of of and of and of has been characterized the of KshAB of tuberculosis. of the two was and the the of KshAB two the of was in of the of KshAB the and of and was was was a and the and and the the of the and the and was of was a a of and was and and by a and containing of of the a of and of in a of a and and and and and was a by the the of and and in and of the two was by in a containing the cluster in and of of of was and and KshB, the was a of and a by containing and was was and was by the a was in and was KshA, of in of containing and by the was a of the was by was and a was a in the and containing protein was the of the containing and was containing and a a was was a of and the was was a of containing and enzyme was a of and and in KshB, of in and ferrous the was a was a of in of and in Purified and was was a a the protein a and of was the was the the containing and protein containing of of of was and protein was by of in a was of a by was and by a a was and and was in a of of containing KshB, and was by of was and in a of enzyme is the of enzyme of the and by of in the of of of in the of the was by of and the and the was the the by the the the and of of a a and a the of and of was containing in a was and was a and was the and a of a of in of and of enzyme a was in in the of and of of the a was the of by of the in of of was the in was a of and the was a of and and of the of and was the was the of the a of in and and containing and in and in of of and in and and the and the and the of the the of and the in the was in the of a was of and the was and the was the of the was the the of KshAB of in the of in the of in a and the in and was was the and the and and the of and of KshAB of by of of of and of by the and of a a and FAD. of and and cluster of and of of of of of the protein in the was KshA, by of and of of of by the of the of the of and of [2Fe-2S] of and a of in the of the of the the of was was KshB, a of the and KshB, in of a was in containing of and a KshAB was the was and the KshAB a and of and a of of of the a the was was the of of in the of of was the of the of and the of by KshAB of the in the and the of of KshAB of of and the was of protein and of is the of the of and in and and and of the of in the of of the the the of is a the of the of by KshAB the of the in the the the a of the of and in the of in the of a of in the of a of a was a a of of the of in of of the molecular a and of and and of of of of is the of the the the in of is the of the the the in in a of two and and the and and was of the and of characterized the Rieske the [2Fe-2S] cluster by a the mononuclear in a the enzyme a of in the is the of of and and the of of the of and the is a of of tuberculosis. of the the in of and and of the Rieske is and in and the is and in and of the and mononuclear of is a the mononuclear and and is is and Rieske of in is the and and Rieske of is of and of and and of the of the Rieske cluster in and and and of the cluster is by and and the is by and in the two the Rieske of of the in the and and in the is and the and the in a of the of KshA, of a by a of the the Rieske in in the Rieske of and and and and the of the of the in the the of the the two the of is two a and and a and and and two a of the the Rieske of the characterized the of is and of of and of the of is a of and is a of the in of and by and and in characterized in of mononuclear and of in in and in in and and in in of is of the by KshA, the and a is the of the and in the Rieske of the the is in the the in and and the the of the two the in and the of the the KshA, the of the of two in KshA, the in the of the in is in the of the and of the KshA, the is the of and the and in the two the and of the and in in it in a the and of and the the the in is of the and and is in in and the mononuclear the of the and of the of two in and and and the the is in the the the in the of the in the of and of is of and mononuclear is by of is by and in a was the in the is has been in of was a the of the was of protein was in of a of a of in of Although was a two in a of of was the the of has been the of KshA, was the two the the mononuclear was the was the in was the is the is the mononuclear in the is of the is in in the by the the of has been in the mononuclear of the in is the of and the of the and in and in of of the of the and the of the the mononuclear the the of in the the of of the the mononuclear and the of is in and and in in in in and in mononuclear is in the the Rieske cluster and mononuclear of of a of the the by of the the of and the of has been implicated in is of is in in KshA, the by the the and the the Rieske of the it is the of in has was in and the oxygenase was a by the of of the Although the oxygenase of was was the enzyme a the of was of KshAB and is and of KshAB was in the of the of KshAB in the KshAB in the catabolic is in the and of of of of KshAB is of of of and of is the of in KshAB the it is the of KshAB is a Although of of of and Mycobacterium and the KshAB it is in tuberculosis. of the of and and of the and in the the of of the the a is the of KshAB is in it the of KshAB a of KshAB the of KshAB in the of is of the and the of and of KshAB was the in of KshAB is of a oxygenase in the cholesterol catabolic pathway the of in of and is the of KshAB the of the of and it is the of KshAB is in of the a and the of the of the the of characterized characterized in and the and characterized in of in the and the of the the of KshA, enzyme a the of the the and a of the of the of the a of is the two in the of is the is the of the characterized the of of the the of the characterized cholesterol is the of the of has been in a of in the of the of a a of in cholesterol in the of and the of the of of enzyme the of Mycobacterium the of the and has a in the of in the a of the of the of is cholesterol catabolic pathway of in cholesterol and in cholesterol is the of the it and of the in the of cholesterol and the pathway the of cholesterol in cholesterol catabolic pathway of of the and the in a it is the of is a of the is by in the of of the of and KshAB 9α-hydroxylase) the of of is a and the the of and is in tuberculosis. the of cholesterol in pathogenesis, a of the of the in the KshAB of is a oxygenase (RO) Rieske of of of of of ferrous a reductase and oxygenase a plant-type cluster and a a by two and two and a mononuclear by two and the in in and Mycobacterium KshAB the of and and the of KshAB is is the of the by enzyme and a of in of in a and two and the reductase the oxygenase, a characterized by the of by been in and the oxygenase two it is the the [2Fe-2S] cluster and a the mononuclear two the the the [2Fe-2S] cluster and the mononuclear of of and the of the the two and a in the of in the of in the of and of has been in the of characterized the of of and of and of has been characterized the of KshAB of tuberculosis. of the two was and the the of KshAB two the of was in of the of KshAB the and of and was was was a and the and and the the of the and the and was of was a a of and was and and by a and containing of of the a of and of in a of a and and and and and was a by the the of and and in and of the two was by in a containing the cluster in and of of of was and and KshB, the was a of and a by containing and was was and was by the a was in and was KshA, of in of containing and by the was a of the was by was and a was a in the and containing protein was the of the containing and was containing and a a was was a of and the was was a of containing and enzyme was a of and and in KshB, of in and ferrous the was a was a of in of and in Purified and was was a a the protein a and of was the was the the containing and protein containing of of of was and protein was by of in a was of a by was and by a a was and and was in a of of containing KshB, and was by of was and in a of enzyme is the of enzyme of the and by of in the of of of in the of the was by of and the and the was the the by the the the and of of a a and a the of and of was containing in a was and was a and was the and a of a of in of and of enzyme a was in in the of and of of the a was the of by of the in of of was the in was a of and the was a of and and of the of and was the was the of the a of in and and containing and in and in of of and in and and the and the and the of the the of and the in the was in the of a was of and the was and the was the of the was the the of KshAB of in the of in the of in a and the in and was was the and the and and the of and and was was was a and the and and the the of the and the and was of was a a of and was and and by a and containing of of the a of and of in a of a and and and and and was a by the the of and and in and of the two was by in a containing the cluster in and of of of was and and KshB, the was a of and a by containing and was was and was by the a was in and was KshA, of in of containing and by the was a of the was by was and a was a in the and containing protein was the of the containing and was containing and a a was was a of and the was was a of containing and enzyme was a of and and in KshB, of in and ferrous the was a was a of in of and in Purified and was was a a the protein a and of was the was the the containing and protein containing of of of was and protein was by of in a was of a by was and by a a was and and was in a of of containing KshB, and was by of was and in a of enzyme is the of enzyme of the and by of in the of of of in the of the was by of and the and the was the the by the the the and of of a a and a the of and of was containing in a was and was a and was the and a of a of in of and of enzyme a was in in the of and of of the a was the of by of the in of of was the in was a of and the was a of and and of the of and was the was the of the a of in and and containing and in and in of of and in and and the and the and the of the the of and the in the was in the of a was of and the was and the was the of the was the the and the in and was was the and the and and the of and of KshAB of by of of of and of by the and of a a and FAD. of and and cluster of and of of of of of the protein in the was KshA, by of and of of of by the of the of the of and of [2Fe-2S] of and a of in the of the of the the of was was KshB, a of the and KshB, in of a was in containing of and a KshAB was the was and the KshAB a and of and a of of of the a the was was the of of in the of of was the of the of and the of by KshAB of the in the and the of of KshAB of of and the was of protein and the of the of by KshAB the of the in the the the a of the of and in the of in the of a of in the of a of a was a a of of the of in of of the molecular a and of and and of of of of is the of the the the in of is the of the the the in in a of two and and the and and was of the and of characterized the Rieske the [2Fe-2S] cluster by a the mononuclear in a the enzyme a of in the is the of of and and the of of the of and the is a of of tuberculosis. of the the in of and and of the Rieske is and in and the is and in and of the and mononuclear of is a the mononuclear and and is is and Rieske of in is the and and Rieske of is of and of and and of the of the Rieske cluster in and and and of the cluster is by and and the is by and in the two the Rieske of of the in the and and in the is and the and the in a of the of KshA, of a by a of the the Rieske in in the Rieske of and and and and the of the of the in the the of the the two the of is two a and and a and and and two a of the the Rieske of the characterized the of is and of of and of the of is a of and is a of the in of and by and and in characterized in of mononuclear and of in in and in in and and in in of is of the by KshA, the and a is the of the and in the Rieske of the the is in the the in and and the the of the two the in and the of the the KshA, the of the of two in KshA, the in the of the in is in the of the and of the KshA, the is the of and the and in the two the and of the and in in it in a the and of and the the the in is of the and and is in in and the mononuclear the of the and of the of two in and and and the the is in the the the in the of the in the of and of is of and mononuclear is by of is by and in a was the in the is has been in of was a the of the was of protein was in of a of a of in of Although was a two in a of of was the the of has been the of KshA, was the two the the mononuclear was the was the in was the is the is the mononuclear in the is of the is in in the by the the of has been in the mononuclear of the in is the of and the of the and in and in of of the of the and the of the the mononuclear the the of in the the of of the the mononuclear and the of is in and and in in in in and in mononuclear is in the the Rieske cluster and mononuclear of of a of the the by of the the of and the of has been implicated in is of is in in KshA, the by the the and the the Rieske of the it is the of in has of KshAB of by of of of and of by the and of a a and FAD. of and and cluster of and of of of of of the protein in the was KshA, by of and of of of by the of the of the of and of [2Fe-2S] of and a of in the of the of the the of was was KshB, a of the and KshB, in of a was in containing of and a KshAB was the was and the KshAB a and of and a of of of the a the was was the of of in the of of was the of the of and the of by KshAB of the in the and the of of KshAB of of and the was of protein and the of the of by KshAB the of the in the the the a of the of and in the of in the of a of in the of a of a was a a of of the of in of of the molecular a and of and of two and and the and and was of the and of characterized the Rieske the [2Fe-2S] cluster by a the mononuclear in a the enzyme a of in the is the of of and and the of of the of and the is a of Rieske of is of and of and and of the of the Rieske cluster in and and and of the cluster is by and and the is by and in the two the Rieske of of the in the and and in the is and the of the of KshA, of a by a of the the Rieske in in the Rieske of and and and and the of the of the in the the of the the two the of is two a and and a and and and two a of the the Rieske of the characterized the of is and of is of the by KshA, the and a is the of the and in the Rieske of the the is in the the in and and the the of the two the in and the of the the KshA, the of the of two in KshA, the in the of the in is in the of the and of the KshA, the is the of and the and in the two the and of the and in in it in a the and of and the the the in is of the and and is in in and the mononuclear the of the and of the of two in and and and the the is in the the the in the of the in the of and of is of and mononuclear is by of is by and in a was the in the is has been in of was a the of the was of protein was in of a of a of in of Although was a two in a of of was the the of has been the of KshA, was the two the the mononuclear was the was the in was the is the is the mononuclear in the is of the is in in the by the the of has been in the mononuclear of the in is the of and the of the and in and in of of the of the and the of the the mononuclear the the in the the Rieske cluster and mononuclear of of a of the the by of the the of and the of has been implicated in is of is in in KshA, the by the the and the the Rieske of the it is the of in has was in and the oxygenase was a by the of of the Although the oxygenase of was was the enzyme a the of was of KshAB and is and of KshAB was in the of the of KshAB in the KshAB in the catabolic is in the and of of of of KshAB is of of of and of is the of in KshAB the it is the of KshAB is a Although of of of and Mycobacterium and the KshAB it is in tuberculosis. of the of and and of the and in the the of of the the a is the of KshAB is in it the of KshAB a of KshAB the of KshAB in the of is of the and the of and of KshAB was the in of KshAB is of a oxygenase in the cholesterol catabolic pathway the of in of and is the of KshAB the of the of and it is the of KshAB is in of the a and the of the of the the of characterized characterized in and the and characterized in of in the and the of the the of KshA, enzyme a the of the the and a of the of the of the a of is the two in the of is the is the of the characterized the of of the the of the characterized cholesterol is the of the of has been in a of in the of the of a a of in cholesterol in the of and the of the of of enzyme the of KshAB was in and the oxygenase was a by the of of the Although the oxygenase of was was the enzyme a the of was of KshAB and is and of KshAB was in the of the of KshAB in the KshAB in the catabolic is in the and of of of of KshAB is of of of and of is the of in KshAB the it is the of KshAB is a Although of of of and Mycobacterium and the KshAB it is in tuberculosis. of the of and and of the and in the the of of the the a is the of KshAB is in it the of KshAB a of KshAB the of KshAB in the of is of the and the of and of KshAB was the in of KshAB is of a oxygenase in the cholesterol catabolic pathway the of in of and is the of KshAB the of the of and it is the of KshAB is in of the a and the of the of the the of characterized characterized in and the and characterized in of in the and the of the the of KshA, enzyme a the of the the and a of the of the of the a of is the two in the of is the is the of the characterized the of of the the of the characterized Although cholesterol is the of the of has been in a of in the of the of a a of in cholesterol in the of and the of the of of enzyme the of and the

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.