Experimental determination of sample stream focusing with fluorescent dye
Bibliographic record
Abstract
This study aims to develop an experimental method for determining the focused-sample stream width in microfluidic chips. The focused-sample stream width in the junction of a cross-linked microchannel is determined by the distance between the two streamlines dividing the focusing sheath streams and the sample stream, which should be insensitive to the sample properties. However, the commonly used FWHM intensity method is dependent on the dye properties and cannot be used to estimate the sample focusing effects very accurately. The proposed method is based upon the fact that the cross-stream concentration profile of the sample stream in a region downstream of the junction matches the Gaussian distribution. Therefore, the width of the focused sample stream can be estimated from the channel widths and the variance and maximum concentration of the experimentally measured concentration profile. To validate this method, fluorescein dye has been used to obtain the concentration profile. It has been shown that the proposed method is independent of the fluorescent dye properties. Comparison between the proposed method, FWHM method, and some reported analytical methods also validates the proposed method.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".