Third Ventricular Injection of Alpha-Bungarotoxin Decreases Pulsatile Luteinizing Hormone Secretion in the Ovariectomized Rat
Bibliographic record
Abstract
The injection of purified alpha-bungarotoxin (alpha-BTX), an antagonist of the muscle nicotinic cholinergic receptors, into the third ventricle (3rd V) of ovariectomized rats decreased the frequency of pulses of luteinizing hormone (LH) but had no significant effect on the amplitude or nadir of LH secretion. Approximately 24 h after the 3rd V injection of alpha-BTX, the pulsatile secretion rate of LH was increased and thus the alpha-BTX effect was reversible. The 3rd V injection of the two nicotinic cholinergic agents, cytisine (10 micrograms) and dihydro-beta-erythroidine, also decreased the frequency of pulses of LH. Cytisine and dihydro-beta-erythroidine inhibited the binding of (125I)alpha-BTX to rat hypothalamic synaptosomes (P2B) and also to frozen coronal sections of the hypothalamus as studied by film autoradiography. Injection of a lower dose of cytisine (1 micrograms) had no effect on LH secretion, but when administered with alpha-BTX into the 3rd V, cytisine significantly reduced the alpha-BTX-induced decreased LH secretion. The injection of cytisine with (125I)alpha-BTX into the 3rd V also decreased in vivo labeling of the (125I)alpha-BTX-binding sites in the hypothalamus of ovariectomized rats. The results suggest that alpha-BTX can inhibit the pulsatile secretion of LH in ovariectomized rats and this inhibition may be mediated by specific subclass of a CNS (central nervous system) nicotinic acetylcholine receptor.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".