Molecular detection and identification of potato isolates of<i>Tobacco rattle virus</i>
Bibliographic record
Abstract
Sixteen isolates of Tobacco rattle virus (TRV) obtained from the United States and Canada were characterized in the present study. Nucleotide-sequence analysis of open reading frame 4 (ORF4) in RNA1 of seven test isolates revealed a high degree of genetic diversity. Potato isolates of TRV clustered in several distinct groups, with isolates from the same geographic region falling into different groups. Phylogenetic analysis of TRV isolates based on nucleotide sequences and on restriction fragment length polymorphism (RFLP) produced similar groupings. TRV was detected in various potato tissues of inoculated, field-grown plants, including progeny tubers, leaves, and roots, by reverse transcription – polymerase chain reaction (RT–PCR), using a pair of TRV-specific primers targeting ORF4. TRV RNA was detected in highly diluted total RNA preparations (1 / 15 625) and in composite tuber samples (200–400 tubers). RT–PCR followed by RFLP analysis was shown to be an efficient procedure for specific and sensitive detection and identification of TRV in potato.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".