Using an oxidized porous silicon interferometer for determination of relative protein binding affinity through non‐covalent capture probe immobilization
Why this work is in the frame
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Bibliographic record
Abstract
Abstract An oxidized porous Si interferometer was used to measure binding of immunoglobulin G (IgG) to an immobilized protein A capture probe. Protein A was non‐covalently immobilized on a thermally oxidized porous Si (PSiO 2 ) sample and exposed to IgG originating from different species. The resulting order of IgG affinity toward the protein A‐coated surface (human > rabbit ≫ sheep IgG) agrees with previous inhibition studies for protein A/IgG binding. No signal change was observed when a protein A coated sample was exposed to bovine serum albumin (BSA), demonstrating that the adsorbed sensing layer sufficiently coats the PSiO 2 surface to prevent non‐specific binding. This strategy is excellent for qualitative measurements of protein binding affinity because it is label‐free, requires minimal sample preparation, and can be implemented using an inexpensive CCD‐based spectrometer coupled to a tungsten lamp. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it