Using an oxidized porous silicon interferometer for determination of relative protein binding affinity through non‐covalent capture probe immobilization
Bibliographic record
Abstract
Abstract An oxidized porous Si interferometer was used to measure binding of immunoglobulin G (IgG) to an immobilized protein A capture probe. Protein A was non‐covalently immobilized on a thermally oxidized porous Si (PSiO2) sample and exposed to IgG originating from different species. The resulting order of IgG affinity toward the protein A‐coated surface (human > rabbit ≫ sheep IgG) agrees with previous inhibition studies for protein A/IgG binding. No signal change was observed when a protein A coated sample was exposed to bovine serum albumin (BSA), demonstrating that the adsorbed sensing layer sufficiently coats the PSiO2 surface to prevent non‐specific binding. This strategy is excellent for qualitative measurements of protein binding affinity because it is label‐free, requires minimal sample preparation, and can be implemented using an inexpensive CCD‐based spectrometer coupled to a tungsten lamp. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".